Thermo Fisher Scientific H3K36me1 Recombinant Rabbit Monoclonal Antibody (14H6L21)
H3K36me1 단일 메틸화 히스톤 H3를 인식하는 재조합 토끼 단클론 항체. Western blot, ICC/IF, Peptide Array에 사용 가능. Expi293 발현, Protein A 정제, 0.5 mg/mL 농도. 높은 특이성과 일관된 로트 간 재현성 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5 µg/mL |
| Peptide Array (Array) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 14H6L21 |
| Immunogen | Methylated peptide (Lys36) corresponding to Human H3 (aa 32–40) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532506 |
Product Specific Information
Since it is highly conserved across species, the antibody may react with many other species.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with other core histone proteins, binds to DNA to form the nucleosome structure.
Histones play central roles in:
- Transcription regulation
- DNA repair
- DNA replication
- Chromosomal stability
Post-translational modifications of histones, including methylation, acetylation, and phosphorylation, influence chromatin structure and gene expression. The N-terminal tail of histone H3 can undergo these modifications, affecting various cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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