Thermo Fisher Scientific H3K4me1 Recombinant Rabbit Monoclonal Antibody (1H4L31)
H3K4me1 표적을 인식하는 재조합 토끼 단클론 항체로, Western blot, ICC/IF, Peptide Array 등에 사용 가능. Expi293 발현 시스템에서 생산되며 높은 특이성과 재현성을 제공. 단백질 A 정제 및 PBS 버퍼에 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| Peptide Array (Array) | 0.25 µg/mL |
| CUT&RUN (C&R) | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 1H4L31 |
| Immunogen | Methylated peptide (Lys4) corresponding to human Histone H3 (aa 2–11) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532504 |
Product Specific Information
Since it is highly conserved across species, the antibody may react with many other species.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to the larger rabbit immune repertoire
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to change the chromatin structure or allow the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the nucleosome core and undergoes several types of post-translational modification, including methylation, acetylation, and phosphorylation, which influence cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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