Thermo Fisher Scientific H3K9me1 Recombinant Rabbit Monoclonal Antibody (9H11L2)
H3K9me1 표적의 재조합 토끼 단클론 항체로, Western blot 및 ICC/IF에 적합합니다. 높은 특이성과 재현성을 제공하며, 로트 간 일관성이 우수합니다. 단백질 A로 정제된 액상 제품으로, PBS 완충액에 보존되고 4°C 또는 -20°C에서 안정적으로 저장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 9H11L2 |
| Immunogen | Methylated peptide (Lys9) corresponding to human Histone H3 (aa 4–11) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532508 |
Product Specific Information
Since it is highly conserved across species, the antibody may react with many other species.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it forms the nucleosome structure by binding to DNA. Histones are essential for transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications of histone H3, such as methylation, acetylation, and phosphorylation, play key roles in controlling chromatin structure and gene expression. The N-terminal tail of histone H3 undergoes these modifications, influencing various cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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