Thermo Fisher Scientific Villin Monoclonal Antibody (SP145)
Villin 단백질을 표적하는 Rabbit Monoclonal Antibody (Clone SP145). Western blot, IHC, ICC/IF, Flow cytometry, Immunoprecipitation에 적합. 인간 및 생쥐 시료 반응성. Protein A/G 정제, PBS buffer에 1% BSA 포함. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP145 |
| Immunogen | Synthetic peptide derived from the C-terminus of human villin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.108 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537927 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with the primary antibody for 10 minutes at room temperature.
Suggested positive controls include colon, small intestine, or kidney tissue.
Target Information
Villin is a cytoskeletal protein associated with the microvillar actin core bundle of gastrointestinal and renal brush border.
It interacts with actin in a Ca²⁺ and phosphoinositide-regulated manner.
Villin serves as a specific marker for adenocarcinomas of the gastrointestinal tract and pancreas, and is also expressed in some Merkel cell tumors and adenocarcinomas of lung, prostate, ovarian, and kidney tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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