Thermo Fisher Scientific PLCG1 Monoclonal Antibody (1F1)
PLCG1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot 및 Immunoprecipitation에 적합합니다. HepG2 세포를 양성 대조군으로 추천하며, 고순도의 인간 PLCγ 단백질을 면역원으로 제작되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunoprecipitation (IP) | 1 µg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F1 |
| Immunogen | Purified, recombinant, human phospholipase C gamma protein expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2163547 |
Product Specific Information
A suggested positive control for this product is HepG2 cells.
Target Information
The mammalian Phospholipase Cγ (PLCγ) family has two closely related proteins, PLCγ1 and PLCγ2. These isozymes share core structural domains and feature a unique array including an additional PH domain, two SH2 domains, and one SH3 domain. Upon stimulation by extracellular signals such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCγ. The activated enzyme catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2), generating the second messengers inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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