Thermo Fisher Scientific PLCG1 Monoclonal Antibody (1F1)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunoprecipitation (IP)

1 µg

-

Product Specifications

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1F1

Immunogen

Purified, recombinant, human phospholipase C gamma protein expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2163547

Product Specific Information

A suggested positive control for this product is HepG2 cells.

Target Information

The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.