Thermo Fisher Scientific NCAM1 Monoclonal Antibody (OTI2A2), TrueMAB

Applications

Western Blot (WB)

• Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

• Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that functions as an adhesive molecule. It is ubiquitously expressed in the nervous system in isoforms ranging from 120–180 kDa. CD56 is found on T cells and NK cells and plays roles in cell migration, axonal growth, pathfinding, and synaptic plasticity.

Polysialic modification reduces CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions with extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal-derived cell lines, tissues, and neoplasms (e.g., retinoblastoma, medulloblastoma, astrocytomas, neuroblastoma). It is also a widely used neuroendocrine marker with high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors.

Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.