Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (eBio20A (20A, A3)), PE, eBioscience
인간 CD152(CTLA-4)에 특이적인 단클론 항체로, Flow Cytometry 분석에 최적화됨. PE 형광단과 결합되어 565/576 nm에서 Excitation/Emission. 4°C 암소 보관, 동결 금지. 연구용으로 사용 가능.
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Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (eBio20A (20A, A3)), PE
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio20A (20A, A3) |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11044911 |
Product Specific Information
The eBio20A monoclonal antibody reacts with human CD152, also known as cytotoxic T lymphocyte antigen-4 (CTLA-4).
CTLA-4 is expressed on activated T cells (and sometimes B cells) and binds CD80 (B7-1) and CD86 (B7-2) with higher affinity than CD28.
CTLA-4 and CD28 deliver opposing signals to T cells: CD28 acts as a costimulator, while CTLA-4 inhibits IL-2 secretion and cellular proliferation.
Intracellular localization of CTLA-4 occurs due to ER retention motifs, leading to accumulation after TCR signaling.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-titrated and validated for intracellular staining and flow cytometric analysis of PHA-stimulated normal human peripheral blood cells.
Use at 5 µL (0.125 µg) per test (final volume 100 µL). Cell number may range from 10⁵–10⁸ cells/test.
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed mainly on CD4+ T cells.
It binds CD80 and CD86 on B cells and dendritic cells with higher affinity than CD28.
Unlike CD28, which enhances T cell activation, CTLA4 inhibits T cell function.
Intracellular CTLA4 is also found in regulatory T cells and may play a role in their function.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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