Thermo Fisher Scientific ROBO3 Polyclonal Antibody, MaxPab

Applications

• Western Blot (WB): 1:500–1:1,000

Product Specifications

Product Specific Information

Sequence of this protein is as follows:
MTPPLQGPRA RFRKKPKALP YRRENSPGDL PPPPLPPPEE EASWALELRA AGSMSSLERE RSGERKAVQA VPLAAQRVLH PDEEAWLPYS RPSFLSRGQG TSTCSTAGSN SSRGSSSSRG SRGPGRSRSQ SRSQSQRPGQ KRREEPR

Target Information

The ROBO3 gene belongs to the Roundabout (ROBO) family, which regulates neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are part of the immunoglobulin transmembrane receptor superfamily. SLIT proteins (1–3) act as ligands for ROBO proteins, mediating processes such as myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, vasculogenesis, and neurogenesis.

ROBO3 has an extracellular domain with five Ig-like loops and three fibronectin type III motifs, a transmembrane region, and a cytoplasmic tail containing three conserved signaling motifs (CC0, CC2, CC3). Unlike other ROBO members, ROBO3 lacks the CC1 motif. It plays a crucial role in axonal navigation at the ventral midline of the neural tube. Loss of ROBO3 in mice leads to failure of commissural axons crossing the midline in the spinal cord and hindbrain. Mutations in ROBO3 are associated with horizontal gaze palsy with progressive scoliosis (HGPPS), an autosomal recessive disorder characterized by congenital absence of horizontal gaze and progressive scoliosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.