Thermo Fisher Scientific beta Tubulin Monoclonal Antibody (AA10)
신경세포에서 주로 발현되는 βIII-튜불린을 인식하는 단클론 항체입니다. Western blot, IHC, ICC 등 다양한 응용에 사용 가능합니다. 고순도의 Protein G 정제 항체로 안정적인 신호 검출을 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | View 2 publications |
| Immunohistochemistry (IHC) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:250 | View 8 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AA10 |
| Immunogen | Synthetic peptide corresponding to beta III tubulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 0.01M HEPES (pH 7.5), 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2532242 |
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize to form microtubules. Beta-III tubulin is primarily expressed in neurons and is implicated in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene can cause congenital fibrosis of the extraocular muscles type 3.
Beta-III tubulin is also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus can be glutamylated, though the function of this modification remains unclear. Tubulin phosphorylation on Ser-172 by CDK1 during cell cycle progression inhibits incorporation into microtubules.
Microtubules, composed of α/β-tubulin dimers (~55 kDa each), are essential cytoskeletal components involved in mitosis, intracellular transport, ciliary and flagellar motion, and structural support. Due to its ubiquitous expression, beta-tubulin is widely used as a loading control in protein detection assays such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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