Thermo Fisher Scientific MTCO1 Monoclonal Antibody (1D6E1A8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 79 publications 79 publications

Immunohistochemistry (IHC)

-

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:5,000

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 4 publications 4 publications

Immunohistochemistry - Free Floating (IHC (Free))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 21 publications 21 publications

Flow Cytometry (Flow)

1:500

-

Miscellaneous PubMed (Misc)

-

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Arthropod, Guinea pig, Human, Mouse, Rat, Zebrafish

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1D6E1A8

Immunogen

Human Mitochondrial Complex IV subunit I

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.99 mg/mL

Purification

Protein A

Storage buffer

PBS with 0.05% BSA, 40% glycerol

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10374492

Product Specific Information

For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal. Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio. In Western blot applications, this antibody detects a band at approximately 40 kDa (predicted MW: 57 kDa). In mouse liver lysate a specific band below 37 kDa was detected.

Recommended positive controls:

ICC/IF - Pig retinal pigment epithelial cells, rat cerebellum primary cells

IHC (P) - Human skeletal muscle and colon tissue, rat pancreas tissue, pig smooth muscle tissue

WB - Mouse, human, bovine and rat heart mitochondria lysate

Flow - HEK-293 cells

Recommended long term storage at -20°C.

Target Information

Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.