Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 647
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 563 / 661 / 737 / 805
• Brilliant Violet 421 / 650 / 786
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue Series (585, 610-70S, 660-120S, 760)
• Pacific Orange
• PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5 / PerCP-eFluor 710
• Super Bright Series (436, 600, 645, 702, 780)
• Request custom conjugation available

Product Specific Information

The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors and activated mature T/B lymphocytes. It associates with CD122 and CD132 to form a high-affinity IL-2 receptor complex. Binding of IL-2 to both receptor classes is inhibited by PC61.5 antibody. CD25 plays a key role in lymphocyte differentiation and activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize antibody titration for best performance.

Brilliant Violet™ 711 (BV711):

• Tandem dye emitting at 713 nm
• Designed for violet (405 nm) laser-equipped cytometers
• Protect from light due to photo-induced oxidation sensitivity

Recommended Buffers:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark. BV711 is not compatible with methanol-based fixation.

Excitation / Emission / Laser:
407 nm / 713 nm / Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD25 (IL2 receptor alpha chain / IL2RA) is a cytokine receptor involved in T and B lymphocyte proliferation. It forms a heterotrimeric complex with IL2RB and IL2RG for high-affinity IL-2 binding. IL2RA expression is monoallelic in mature thymocytes, regulating gene expression precision. IL-2 acts as a growth and differentiation factor and regulates apoptosis. It stimulates B-cell proliferation, enhances NK cell activity, and inhibits granulocyte macrophage colony formation. Mutations in IL2RA may cause immune dysfunctions such as ulcerative colitis-like disease.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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