Thermo Fisher Scientific NFkB p65 Recombinant Rabbit Monoclonal Antibody (6D6R8)
NFkB p65 단백질을 인식하는 재조합 토끼 단일클론 항체로, WB, IHC, ICC, ELISA, IP, ChIP 등 다양한 응용에 적합함. 높은 특이성과 재현성을 제공하며, 인간 및 설치류 등 여러 종에 반응. HEK293 세포에서 발현된 고순도 항체로 연구용에 최적화됨.
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Thermo Fisher Scientific NFkB p65 Recombinant Rabbit Monoclonal Antibody (6D6R8)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:60,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:800 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–500 µg extracts |
| ChIP assay (ChIP) | 5 µg antibody for 10–15 µg chromatin |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 6D6R8 |
| Immunogen | Synthetic peptide within amino acids 450–551 of human NF-kB p65/RelA (NP_068810.3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3093682 |
Product Specific Information
Immunogen Sequence:
LGALLGNSTD PAVFTDLASV DNSEFQQLLN QGIPVAPHTT EPMLMEYPEA ITRLVTGAQR PPDPAPAPLG APGLPNGLLS GDEDFSSIAD MDFSALLSQI SS
Target Information
Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene located on chromosome 11 in humans. Also known as RelA, this protein belongs to the Rel family and forms a heterodimer with p50 or p52 subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells).
NF-kB p65 plays a crucial role in transcriptional regulation, particularly in the expression of the immunoglobulin kappa gene in mature B-lymphoid cells. Activation occurs via TNF-alpha signaling, leading to phosphorylation and degradation of IκB, allowing NF-kB translocation to the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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