Thermo Fisher Scientific Methyl-Retinoblastoma (Lys860) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

ELISA (ELISA)

1:10,000-1:50,000

-

Immunoprecipitation (IP)

1.0 µg/mL

-

Dot blot (DB)

0.25-1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Retinoblastoma [Monomethyl Lys860] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a methylated and acetylated synthetic peptide surrounding lysine 860 of human RB1 conjugated to Keyhole Limpet Hemocyanin (KLH). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Rb,uniProtId:P06400-1,ncbiNodeId:9606,antigenRange:860,antigenLength:928,antigenImageFileName:600-401-GZ4_Rb_P06400-1_Rabbit.svg,antigenImageFileNamePDP:600-401-GZ4_Rb_P06400-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.99 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human Retinoblastoma. This antibody reacts with human RB1 surrounding aa860 and is specific for K860 Me1. Blast analysis of the sequence of the immunogen shows 100% identity with human and 92% identity to rat and mouse.

Target Information

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.