Thermo Fisher Scientific HEY1 Polyclonal Antibody
HEY1 단백질을 인식하는 토끼 폴리클로날 항체로, WB, IHC, ELISA에 사용 가능. 인간과 생쥐에 반응하며, Notch 신호전달 관련 연구에 적합. 고순도의 친화 크로마토그래피 정제 항체로 안정적인 액상 형태 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-HEY1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human HEY1 conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.06 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human HEY1. A BLAST analysis suggests cross-reactivity with mouse (100% homology) and dog/cattle (91.7% homology) based on the immunizing sequence.
Target Information
Hairy/enhancer-of-split related with YRPW motif protein 1 (HEY1) belongs to the HEY family and is a Notch Signaling Pathway Protein. It contains one basic helix-loop-helix (bHLH) domain and one Orange domain. It acts as a downstream effector of Notch signaling, potentially required for cardiovascular development. HEY1 functions as a transcriptional repressor binding preferentially to the canonical E-box sequence 5'-CACGTG-3', repressing transcription by cardiac activators GATA4 and GATA6. It is also expressed during nervous system development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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