Thermo Fisher Scientific XAF1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptides, KLH conjugated, corresponding to residues 201-218 and 301-317 of human XaF1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:XAF1,uniProtId:Q6GPH4-1,ncbiNodeId:9606,antigenRange:201-218|301-317,antigenLength:301,antigenImageFileName:PA1-41099_XAF1_Q6GPH4-1_Rabbit.svg,antigenImageFileNamePDP:PA1-41099_XAF1_Q6GPH4-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2216764

Product Specific Information

Suggested positive control: antigen standard for XAF1 (transient overexpression lysate), human liver tissue or protein.

Target Information

The inhibitors of apoptosis (IAP) proteins prevent cell death both in vitro and in vivo by virtue of inhibition of distinct caspases. The X-linked Inhibitor of Apoptosis, XIAP, is a key member of the IAP family of proteins. The caspase inhibiting activity of XIAP is negatively regulated by at least two XIAP-interacting proteins, XIAP-associated factor 1 (XAF1) and Smac/DIABLO. XAF1 binds to XIAP and antagonizes caspase inhibitory activities of XIAP. Expression of XAF1 triggers a redistribution of XIAP from the cytosol to the nucleus. XAF1 is ubiquitously expressed in normal tissues, but is present at low or undetectable levels in many different cancer cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.