Thermo Fisher Scientific XAF1 Polyclonal Antibody
XAF1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot 및 IHC(P)에서 검증됨. Human 및 Mouse 반응성. 단백질 G 정제, 액상 형태, 0.5 mg/mL 농도. 연구용으로만 사용.
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Thermo Fisher Scientific XAF1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:500 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptides, KLH conjugated, corresponding to residues 201–218 and 301–317 of human XAF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2216764 |
Product Specific Information
Suggested positive control: antigen standard for XAF1 (transient overexpression lysate), human liver tissue or protein.
Target Information
The inhibitors of apoptosis (IAP) proteins prevent cell death both in vitro and in vivo by inhibiting distinct caspases. The X-linked Inhibitor of Apoptosis (XIAP) is a key member of the IAP family. XIAP’s caspase-inhibiting activity is negatively regulated by XIAP-associated factor 1 (XAF1) and Smac/DIABLO.
XAF1 binds to XIAP and antagonizes its caspase inhibitory activities, triggering redistribution of XIAP from the cytosol to the nucleus. XAF1 is expressed ubiquitously in normal tissues but is often reduced or undetectable in various cancer cell lines.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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