Thermo Fisher Scientific SARS Coronavirus Nucleocapsid Polyclonal Antibody
SARS-CoV 핵단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC 등 다양한 응용에 사용 가능. SARS-CoV-2 N 단백질과 77% 상동성. 항원 친화 크로마토그래피로 정제된 액상 항체로, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:2,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 8 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | – |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:10–1:500 | View 5 publications |
| In vitro Assay (IV) | – | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Virus |
| Published Species | Virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues 399–411 of putative SARS nucleocapsid |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1087200 |
Product Specific Information
- The immunogen is 77% (10/13) homologous to SARS-CoV-2 (COVID-19) nucleocapsid protein.
- Cross-reactivity has not been empirically tested.
- Suggested positive control: transfected mouse melanoma cell lysate.
Target Information
Severe Acute Respiratory Syndrome (SARS) is an emerging disease characterized by atypical pneumonia, attributed to a novel coronavirus (SARS-CoV). The nucleocapsid protein (N) is one of the structural proteins of SARS-CoV (N, S, sE, M) and is the most abundant protein in virus-infected cells.
The SARS-CoV-2 S gene has 76% amino acid similarity to the SARS-CoV S gene. Reports indicate that although the SARS-CoV-2 nucleocapsid protein shares structural similarity with previously described coronavirus N proteins, their surface electrostatic potential characteristics are distinct.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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