Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV Series, Brilliant Violet Series, eFluor 506, FITC, Functional Grade, NovaFluor Series, PE, PE-Cyanine Series, PE-eFluor 610, PerCP-Cyanine5.5, Super Bright Series, and custom conjugation available.

Product Specific Information

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed on all hematopoietic cells except mature erythrocytes and platelets. It possesses tyrosine phosphatase activity crucial for lymphocyte activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm (Violet laser). Ensure instrument compatibility.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells (~10% of lymphocyte surface proteins). Absent in non-hematopoietic tissues, CD45 is critical for lymphocyte development and antigen signaling through regulation of Src-family kinases. Multiple isoforms arise via alternative splicing, differing in extracellular domains. CD45 regulates T- and B-cell receptor signaling, cytokine receptor pathways, and integrin-mediated signaling, influencing immune responses and apoptosis regulation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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