Thermo Fisher Scientific CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 555
• NovaFluor Blue 660-120S
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alpha IIb). CD41 is expressed on platelets, megakaryocytes, and hematopoietic progenitors in the embryo, fetus, and adult. It associates with CD61 (gpIIIa, integrin beta III) to form a receptor essential for platelet function, including binding of adhesion molecules such as fibrinogen, fibronectin, and vitronectin.

The SLAM-family markers CD48 and CD150 are used to identify hematopoietic stem cells (HSC). CD150⁺CD48⁻ bone marrow cells show high efficiency in long-term multi-lineage reconstitution, which is enhanced when CD41⁺ cells are excluded. Thus, CD150⁺CD48⁻CD41⁻ expression efficiently identifies HSCs.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry of mouse platelets at ≤0.5 µg/test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal results.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®. It emits at 445 nm and is excited with the Violet laser (405 nm). Ensure instrument compatibility.

• Excitation: 405 nm
• Emission: 445 nm
• Laser: Violet
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD41 (platelet glycoprotein IIb, ITGA2B) consists of two subunits (120 kDa α and 23 kDa β) that interact with CD61 in the presence of calcium to form a functional adhesive receptor. CD41 mediates platelet aggregation and binds to von Willebrand factor, fibrinogen, fibronectin, and vitronectin upon vascular injury. It is mainly expressed in the megakaryocyte-platelet lineage and during early hematopoietic differentiation.

Associated Diseases: Glanzmann Thrombasthenia, Platelet-type 16 bleeding disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지