Thermo Fisher Scientific H3K27me3 Recombinant Mouse Monoclonal Antibody (BT164)
H3K27me3 표지를 인식하는 Thermo Fisher BT164 마우스 단클론 항체로, 인간 시료에 반응합니다. WB, IHC, ICC, ELISA, IP 등 다양한 응용에 적합하며, HEK293 세포에서 발현된 recombinant 형입니다. Protein A로 정제되어 안정적인 액상 형태로 제공됩니다.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- Immunocytochemistry (ICC/IF): Assay-dependent
- ELISA: Assay-dependent
- Immunoprecipitation (IP): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BT164 |
| Immunogen | BT164 is a mice-derived lupus antibody established by selection on apoptotic chromatin. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
The epitope of BT164 was mapped in the N-terminal tail of histone H3 (27-KSAPAT-32) and includes apoptosis-induced trimethylation of K27.
Target Information
H3K27me3 is the epigenetic mark where histone H3 is trimethylated at lysine residue 27. It serves as an epigenetic silencing mark associated with downregulation of nearby genes and is often found in heterochromatin regions.
H3K27me3 is mainly located at inactive gene promoters and opposes the activating mark H3K4me3. It is closely associated with developmental regulators.
EZH2 (PRC2 complex) acts as the histone methyltransferase (writer) for H3K27me3, while UTX and JMJD3 function as demethylases (erasers).
Regulation of H3K27me3 is critical for gene expression control, and its dysregulation has been implicated in various diseases including cancer.
H3K27me3 antibodies are widely used for Western blot and ChIP analyses to study changes in levels and occupancy of this mark.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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