Thermo Fisher Scientific Myosin Skeletal Muscle Monoclonal Antibody (414)
골격근 미오신을 인식하는 Mouse IgM 단일클론 항체로 다양한 종(Chicken, Human, Mouse, Rabbit, Rat)에 반응. Western blot, IHC, ICC 등 여러 응용 가능. 비결합형 액상 형태로 4°C에서 보관. 연구용 전용 제품.
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:5–1:10 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:5–1:10 | - |
| Immunocytochemistry (ICC/IF) | 1:10 | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rabbit, Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 414 |
| Immunogen | Chicken skeletal muscle cell preparation |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Tissue culture supernatant |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2147020 |
Product Specific Information
Mab 414 reacts with myosin-containing elements in most cell types.
Antigen localization: Cytoplasm.
In Western blot experiments, the antibody reacts with a 75 kDa part of the heavy chain (epitope located on a myosin-common sitechain) and shows no reaction with light chains.
It is not reactive with alpha-cardiac myosin.
Positive control: Muscle, myofibrils (chicken).
Target Information
Myosin is a major contractile protein that converts chemical energy into mechanical energy through ATP hydrolysis.
It is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains.
Myosin heavy chains are encoded by a multigene family.
In mammals, at least 10 different MYH isoforms have been described from striated, smooth, and nonmuscle cells.
These isoforms show expression that is spatially and temporally regulated during development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일: MA1-35718_Myosin_Skeletal_Muscle_P13538-1_House_mouse.svg, MA1-35718_Myosin_Skeletal_Muscle_P13538-1_House_mouse_PDP.jpeg)
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