Thermo Fisher Scientific Myosin Skeletal Muscle Monoclonal Antibody (414)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:5-1:10

-

Immunohistochemistry (Frozen) (IHC (F))

1:5-1:10

-

Immunocytochemistry (ICC/IF)

1:10

-

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rabbit, Rat

Published species

Rat

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

414

Immunogen

Chicken skeletal muscle cell preparation if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Myosin Skeletal Muscle,uniProtId:P13538-1,ncbiNodeId:9031,antigenRange:1-1939,antigenLength:1939,antigenImageFileName:MA1-35718_Myosin_Skeletal_Muscle_P13538-1_House_mouse.svg,antigenImageFileNamePDP:MA1-35718_Myosin_Skeletal_Muscle_P13538-1_House_mouse_PDP.jpeg,sortOrder:5}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2147020

Product Specific Information

Mab 414 reacts with myosin-containing elements in most cell types. Antigen localization: cytoplasm In Western blot experiments the antibody reacts with a 75kD part of the heavy chain (epitope location on a myosin-common sitechain), no reaction with light chains. It is not reactive with alpha-cardiac myosin. Positive control: muscle, myofibrils (chicken)

Target Information

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.