Thermo Fisher Scientific Fluorescein Polyclonal Antibody, HRP
Thermo Fisher Scientific의 HRP 결합 Fluorescein Polyclonal Antibody는 IHC, ELISA, IP, Dot blot 등 다양한 면역분석에 적합합니다. Goat IgG 기반의 고순도 항체로 안정적인 형광 검출을 제공합니다. 연구용으로만 사용되며 장기 보관 시 -20°C에서 안정성을 유지합니다.
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Thermo Fisher Scientific Fluorescein Polyclonal Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:200,000 |
| Immunoprecipitation (IP) | 1:5,000–1:10,000 |
| Dot blot (DB) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fluorescein conjugated to Goat IgG |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, and Fluorescein conjugated IgG.
Target Information
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light, causing molecules to emit visible yellow-green light. This emission ceases upon removal of the excitation light.
Fluorochrome labeling provides rapid and accurate localization of antigen-antibody interactions when one reactant is part of a cell, tissue, or biological structure.
FITC is a common marker for antibodies in immunofluorescent techniques because conjugation to proteins is simple and generally preserves biological activity.
It is widely used as a hapten to label different proteins. Anti-FITC antibodies can isolate FITC-labeled cells using magnetic microbeads or remove background signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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