Thermo Fisher Scientific Fluorescein Polyclonal Antibody, AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunohistochemistry (IHC)

1:200-1:1,000

-

ELISA (ELISA)

1:2,000-1:10,000

-

Immunoprecipitation (IP)

1:500-1:2,500

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fluorescein conjugated to Goat IgG

Conjugate

AP AP AP

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.05M tris HCl, pH 8, with 50% glycerol, 10mg/mL BSA, 0.15M NaCl, 0.0001M ZnCl2, 0.001M MgCl2

Contains

0.01% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, anti-Alkaline Phosphatase (calf intestine) and Fluorescein conjugated IgG.

Target Information

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.