Thermo Fisher Scientific GST Polyclonal Antibody, FITC
FITC 결합형 GST 폴리클로날 항체로 Western blot, ICC/IF, ELISA, IP 등 다양한 응용 가능. Schistosoma japonicum 유래 GST 항원을 인식하며, 염소 IgG 기반. 형광 특성 498/517 nm, 고순도 친화 크로마토그래피 정제. 연구용으로 단백질 상호작용 분석에 적합.
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Thermo Fisher Scientific GST Polyclonal Antibody, FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,500 |
| ELISA | >1:20,000 |
| Immunoprecipitation (IP) | >1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Glutathione-S-Transferase (Schistosoma japonicum) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, as well as purified and partially purified Glutathione-S-Transferase (Schistosoma japonicum).
Cross-reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Target Information
GST (Glutathione S-transferase) is a 26 kDa protein present in both eukaryotes and prokaryotes, catalyzing a variety of reactions.
Glutathione S-transferase is used to create the GST gene fusion system, allowing purification of GST-fusion proteins via high affinity for glutathione.
Fusion proteins provide an important biological assay for direct protein-to-protein interactions.
The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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