Thermo Fisher Scientific Biotin Polyclonal Antibody, HRP
HRP 결합된 Biotin 폴리클로날 항체로 IHC, ELISA, IP 등 다양한 면역실험에 사용 가능. 염소 유래 IgG 항체이며 KLH에 결합된 Biotin을 면역원으로 함. 친화 크로마토그래피로 정제되어 높은 특이성과 안정성을 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:140,000 |
| Immunoprecipitation (IP) | 1:3,000–1:15,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Biotin conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, biotinylated bovine serum albumin, and Biotinylated IgG.
Target Information
Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species.
Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a molecular weight of approximately 244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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