Thermo Fisher Scientific Protein C Recombinant Mouse Monoclonal Antibody (HPC-4)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:200

-

ELISA (ELISA)

10 µg/mL

-

Inhibition Assays (IA)

Assay-dependent

-

Affinity Purification (AP)

Assay-dependent

-

Product Specifications

Species Reactivity

Baboon, Human

Host/Isotype

Mouse / IgG1, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

HPC-4

Immunogen

This antibody was raised by immunising mice with human protein C.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Specifically binds to a specific twelve peptide sequence (EDQVDPRLIDGK) in the activation region of the Protein C (spanning the thrombin cleavage site). Does not react with activated protein C. Has been shown to be cross-reactive with baboon Protein C but not with bovine or mouse Protein C.

Target Information

Protein C is a vitamin K-dependent plasma glycoprotein that is cleaved to its activated form by the thrombin-thrombomodulin complex. Protein C in its activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62 kDa proenzyme is activated by thrombin bound to an endothelial surface receptor and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55 kDa and the normal concentrations in human plasma is approximately 1-3 ng/mL because of the very fast turnover, the proenzyme concentration is approximately 3 µg/mL. Mutations in the Protein C gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.