Thermo Fisher Scientific Nitrotyrosine Monoclonal Antibody (HM.11), Biotin

Applications and Tested Dilution

Product Specifications

Product Specific Information

The monoclonal antibody HM.11 recognizes nitrotyrosine, both as the free amino acid and within proteins containing nitrotyrosine.

Target Information

Nitrotyrosine is a relatively stable product formed from various reaction pathways, most notably the reaction of peroxynitrite (derived from superoxide and nitric oxide radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration reactions on proteins, resulting in inactivation of certain housekeeping enzymes (e.g., alpha1-antiproteinase) and endogenous antioxidant enzymes such as catalase and superoxide dismutase (SOD).

Nitrotyrosine serves as an indicator of cell damage and inflammation, as well as nitric oxide (NO) production. Measuring nitrotyrosine concentration can serve as a marker for NO-induced cellular damage. It has been implicated in the pathogenesis of several inflammatory, infectious, and degenerative diseases, including Alzheimer’s and Parkinson’s disease, multiple sclerosis, amyotrophic lateral sclerosis (ALS), asthma, atherosclerosis, and conditions related to endothelial injury.

At the protein level, tyrosine nitration can lead to loss or alteration of protein function, as demonstrated for oncogenic protein p53 and mitochondrial manganese-dependent superoxide dismutase (MnSOD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.