Thermo Fisher Scientific IL-2 Polyclonal Antibody

Applications

Tested Dilution

Publications

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)

-

Neutralization (Neu)

0.25-1.5 µg/mL

-

Product Specifications

Species Reactivity

Dog

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant canine IL-2 Ala21-Thr155 with a Cys147Ser substitution if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-2,uniProtId:Q29416-1,ncbiNodeId:9615,antigenRange:21-155,antigenLength:155,antigenImageFileName:PA5-46915_IL-2_Q29416-1_Goat.svg,antigenImageFileNamePDP:PA5-46915_IL-2_Q29416-1_Goat_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2607641

Product Specific Information

In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant human IL-2, recombinant mouse IL-2, recombinant rat IL-2, recombinant feline IL-2, recombinant cotton rat IL-2, recombinant bovine IL-2, recombinant equine IL-2, and recombinant porcine IL-2 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.