Thermo Fisher Scientific NGAL Polyclonal Antibody
NGAL 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 폴리클로날 항체입니다. Western blot, IHC, IP 등에 사용 가능하며, 마우스 시료에 반응합니다. 고순도 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.25–5 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 3–15 µg/mL |
| Immunoprecipitation (IP) | 25 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2/NGAL (Gln21–Asn200) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609490 |
Product Specific Information
- In direct ELISAs, approximately 50% cross-reactivity with recombinant rat Lipocalin-2/NGAL is observed.
- Approximately 10% cross-reactivity with recombinant human Lipocalin-2/NGAL is observed.
- Less than 5% cross-reactivity with recombinant human Lipocalin-1 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
NGAL (Neutrophil gelatinase-associated lipocalin, also known as lipocalin 2, siderocalin, or neutrophil lipocalin) is a 25 kDa glycosylated single-chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules.
NGAL is released by activated neutrophils, can form dimers and higher-order oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B).
Low-level expression of NGAL in various epithelia may increase during inflammation or cancer. Expression in the kidney is dramatically elevated by ischemic or nephrotoxic injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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