Thermo Fisher Scientific NGAL Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25-5 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

3-15 µg/mL

-

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2/NGAL Gln21-Asn200 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NGAL,uniProtId:P11672-1,ncbiNodeId:10090,antigenRange:21-200,antigenLength:200,antigenImageFileName:PA5-46916_NGAL_P11672-1_Goat.svg,antigenImageFileNamePDP:PA5-46916_NGAL_P11672-1_Goat_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609490

Product Specific Information

In direct ELISAs, approximately 50% cross-reactivity with recombinant rat Lipocalin-2/NGAL is observed, approximately 10% cross-reactivity with recombinant human (rh) Lipocalin-2/NGAL is observed, and less than 5% cross-reactivity with rhLipocalin-1 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.