Thermo Fisher Scientific RbAp48 Monoclonal Antibody (11G10)
RbAp48 단백질을 인식하는 Mouse Monoclonal Antibody (Clone 11G10). Western blot, IHC, ICC, IP, ChIP 등 다양한 어플리케이션에 사용 가능. Protein G로 정제된 액상 항체로, 1 mg/mL 농도. Human, Mouse, Rat 반응성. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:500–1:1,000 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| ChIP assay (ChIP) | Assay-dependent | - |
| Functional Assay (Functional) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11G10 |
| Immunogen | The complete coding region (amino acids 1–425) of RbAp48 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_560910 |
Product Specific Information
- Positive control: MOLT4, HeLa, LB22
- For ICC, a permeabilization step is recommended.
- Use at a dilution of 1:500–1:1000 in PBST containing 2% BSA and 10% normal serum at 4°C overnight.
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
RbAp48-11G10 recognizes full-length RbAp48, a 48 kDa nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb).
It belongs to the WD (Trp-Asp) repeat family of proteins and is one of the three subunits of CAF-1.
RbAp48 can bind to histone H4 and is a subunit of human histone deacetylase HD1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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