Thermo Fisher Scientific RbAp48 Monoclonal Antibody (11G10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:500-1:1,000

-

Immunoprecipitation (IP)

Assay-dependent

-

ChIP assay (ChIP)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

11G10

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560910

Product Specific Information

Positive control: MOLT4, HeLa, LB22. For ICC a permeabilization step is recommended; use at a dilution of 1:500-1:1000 in PBST containing 2%BSA and 10% normal serum at 4°C overnight.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

RbAp48-11G10 recognizes full length RbAp48, a 48 kDa nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.