Thermo Fisher Scientific RAD51 Monoclonal Antibody (14B4)
RAD51 단백질을 인식하는 Mouse Monoclonal Antibody (Clone 14B4)로, WB, IHC, ICC/IF, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제된 액상형 제품이며, 인간·마우스·랫 등에서 반응. DNA 복구 및 상동 재조합 연구에 활용 가능.
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Thermo Fisher Scientific RAD51 Monoclonal Antibody (14B4)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 2 publications |
| Flow Cytometry (Flow) | - | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | - |
| in situ PLA (PLA) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14B4 |
| Immunogen | Full length Rad51 protein (amino acids 1–338) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_560832 |
Product Specific Information
A suggested positive control for this product is T24.
Target Information
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recombination.
It binds to single- and double-stranded DNA and exhibits DNA-dependent ATPase activity.
RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template.
It forms nucleoprotein filaments in an ATP-dependent manner which are essential for the homology search and strand exchange.
Mutations in the gene can result in breast cancer, mirror movements 2, and Fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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