Thermo Fisher Scientific RAD51 Monoclonal Antibody (14B4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

in situ PLA (PLA)

Assay-dependent

-

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

14B4

Immunogen

Full length Rad51 protein (amino acids 1-338) expressed in E. coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAD51,uniProtId:Q06609-1,ncbiNodeId:9606,antigenRange:1-338,antigenLength:339,antigenImageFileName:MA1-23271_RAD51_Q06609-1_House_mouse.svg,antigenImageFileNamePDP:MA1-23271_RAD51_Q06609-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560832

Product Specific Information

A suggested positive control for this product is T24.

Target Information

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.