Thermo Fisher Scientific RAD50 Monoclonal Antibody (13B3)
RAD50 단백질을 인식하는 Mouse IgG1 단일클론 항체로, DNA 이중가닥 손상 복구 연구에 활용됩니다. Western blot, IHC, ICC, IP 등 다양한 응용에 적합하며, HeLa 세포를 양성 대조로 권장합니다. 단기 4°C, 장기 -20°C 보관.
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Thermo Fisher Scientific RAD50 Monoclonal Antibody (13B3)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| Immunoprecipitation (IP) | Assay-dependent | View 1 publication |
| In vitro Assay (IV) | Assay-dependent | – |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 13B3 |
| Immunogen | Amino acids 1–425 of Rad50 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.7 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_560822 |
Product Specific Information
Recommended positive controls: HeLa, HeLa nuclear extract.
Target Information
Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double-strand break repair.
Rad50 forms a complex with Mre11 and p95 (Nibrin) to mediate the double-strand break repair process.
Upon DNA damage, Rad50 and Mre11 redistribute and co-localize within the nucleus of damaged cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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