Thermo Fisher Scientific MCP-1 Polyclonal Antibody, PeproTech
Human MCP-1(CCL2)에 특이적인 Rabbit Polyclonal Antibody로, Western blot 및 ELISA에 최적화되어 있습니다. 항원 친화 크로마토그래피로 정제되었으며, 고감도 검출이 가능합니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- 500-P34-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific MCP-1 Polyclonal Antibody, PeproTech
Thermo Fisher Scientific MCP-1 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 2 publications |
| ELISA | 0.5–2.0 µg/mL | View 4 publications |
| Neutralization (Neu) | – | View 2 publications |
| In vitro Assay (IV) | – | View 4 publications |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
| Immunostaining (IS) | – | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human MCP-1 (CCL2) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930473 |
Product Specific Information
AA Sequence of recombinant protein:
QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MCP-1 (CCL2). Anti-Human MCP-1 (CCL2)-specific antibody was purified by affinity chromatography employing an immobilized Human MCP-1 (CCL2) matrix.
Sandwich ELISA:
To detect Human MCP-1 (CCL2) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. In conjunction with PeproTech Biotinylated Anti-Human MCP-1 (CCL2) (500-P34Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hMCP-1 (CCL2).
Western Blot:
To detect hMCP-1 (MCAF) by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant hMCP-1 (MCAF) is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. The encoded protein is structurally related to the CXC subfamily of cytokines, characterized by two cysteines separated by a single amino acid. MCP-1 displays chemotactic activity for monocytes and basophils, but not for neutrophils or eosinophils. It is implicated in diseases with monocytic infiltrates such as psoriasis, rheumatoid arthritis, and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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