Thermo Fisher Scientific MCP-1 Polyclonal Antibody, PeproTech

Thermo Fisher Scientific MCP-1 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MCP-1 (CCL2). Anti-Human MCP-1 (CCL2)-specific antibody was purified by affinity chromatography employing an immobilized Human MCP-1 (CCL2) matrix.

Sandwich ELISA:
To detect Human MCP-1 (CCL2) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. In conjunction with PeproTech Biotinylated Anti-Human MCP-1 (CCL2) (500-P34Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hMCP-1 (CCL2).

Western Blot:
To detect hMCP-1 (MCAF) by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant hMCP-1 (MCAF) is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. The encoded protein is structurally related to the CXC subfamily of cytokines, characterized by two cysteines separated by a single amino acid. MCP-1 displays chemotactic activity for monocytes and basophils, but not for neutrophils or eosinophils. It is implicated in diseases with monocytic infiltrates such as psoriasis, rheumatoid arthritis, and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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