Thermo Fisher Scientific CCL5 (RANTES) Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific CCL5 (RANTES) Polyclonal Antibody, Biotin

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human RANTES (CCL5). Anti-Human RANTES (CCL5)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hRANTES by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human RANTES (500-P36) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hRANTES.

Western Blot:
To detect hRANTES by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hRANTES is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P36BT-1MG will be provided as 2 × 500 µg.

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes.
CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It induces histamine release from basophils and activates eosinophils.
This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells and acts as a natural ligand for CCR5, suppressing replication of R5 strains of HIV-1.
Alternative splicing results in multiple transcript variants encoding different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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