Thermo Fisher Scientific Mono-Methyl-Histone H3 (His39) Polyclonal Antibody
Mono-methyl Histone H3 (His39) 특이적 Rabbit Polyclonal Antibody로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, IP에 사용 가능하며, 항원 친화 크로마토그래피로 정제된 고순도 제품입니다. 연구용으로 핵 내 히스톤 변형 분석에 적합합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide containing GVKKP-H(Me1)-RYKPG, where Me1-K corresponds to mono-methyl Histidine 39 of human histone H3 (amino acids 35–45) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: 3T3 Nuclear Extract
- Cellular location: Nucleus
Target Information
Histones are basic nuclear proteins that, together with DNA, form nucleosomes in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of H2A, H2B, H3, and H4.
Methylation of specific histone residues regulates epigenetic transitions in chromatin.
- Methylation at H3 lysine 4, 36, and 79: associated with gene activation
- Methylation at H3 lysine 9, 27, and H4 lysine 20: associated with heterochromatin and gene repression
Modified lysine residues can be mono-, di-, or tri-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) marks actively transcribed chromatin.
This antibody specifically recognizes histone H3 tri-methylated at K27 and reacts with mammalian and other species (D. melanogaster, Arabidopsis, Chicken, Xenopus) where this modification is present.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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