Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), Super Bright 600, eBioscience
마우스 CD31(PECAM-1)을 인식하는 390 클론 단일클론 항체로, Super Bright 600 형광체로 결합됨. 유세포분석에 최적화되어 있으며, 405 nm 레이저에서 여기되어 600 nm에서 방출. 4°C 암소 보관, 비동결. 연구용으로만 사용 가능.
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- 63-0311-8x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), Super Bright 600, eBioscience
Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), Super Bright 600, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | - | View 13 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 10 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 46 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 600 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 390 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2717030 |
Available Formats
- Unconjugated
- APC
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 711
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 610
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 645
- Request custom conjugation
Product Specific Information
Description:
The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed by endothelial cells and at low levels on leukocytes and platelets. CD31 mediates homotypic adhesion and plays roles in cell-cell and cell-matrix interactions and signal transduction.
Applications Reported:
Suitable for flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.125 µg/test (100 µL final volume). Cell count per test: 10⁵–10⁸ cells. Antibody titration recommended for optimal results.
Fluorochrome Information:
Super Bright 600 is excited by the violet laser (405 nm) and emits at 600 nm; use a 610/20 bandpass filter. Ensure instrument compatibility.
Usage Recommendations:
- When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
- Protect vial and stained samples from light (photo-induced oxidation sensitivity).
- Fixed samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
Excitation/Emission: 405 nm / 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling through ITIM-mediated phosphorylation, providing docking sites for protein-tyrosine phosphatases. It is mainly expressed at endothelial junctions and mediates homophilic binding crucial for cell-cell interactions. CD31 functions in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immune regulation, and thrombosis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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