Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), PE-Cyanine7, eBioscience
Mouse 및 Rat IL-17A를 특이적으로 인식하는 단클론 항체로, Flow Cytometry에 최적화된 PE-Cyanine7 형광 표지 제품입니다. 염증성 사이토카인 분석 및 Th17 세포 연구에 적합하며, 높은 특이성과 안정적인 형광 성능을 제공합니다.
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Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), PE-Cyanine7, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 108 publications |
| Functional Assay (Functional) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio17B7 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10717952 |
Available Formats
- Alexa Fluor 488
- APC
- Biotin
- Brilliant UV 395, 615, 661, 737, 805
- Brilliant Violet 421, 650, 711, 786
- eFluor 450, 506, 660
- FITC
- PE
- PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The eBio17B7 antibody reacts with mouse and rat IL-17A without recognizing IL-17F. IL-17A is a CD4+ T cell-derived cytokine involved in inflammatory responses and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
Human IL-17A exists as glycosylated 20–30 kD homodimers produced by activated CD4+ T-cells and promotes secretion of IL-6, IL-8, G-CSF, and PGE2 from epithelial and fibroblastic cells.
IL-23-dependent, IL-17A-producing CD4+ T cells (Th17 cells) represent a distinct subset from Th1 and Th2 cells. IFN-γ and IL-4 negatively regulate Th17 cell generation.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on restimulated Th17-polarized mouse splenocytes using Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00). Recommended ≤0.5 µg/test.
Cell number: 10⁵–10⁸ cells/test. Optimize antibody titration for best results.
Compatible with Foxp3 buffer system (Product # 00-5523-00).
Light Sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57).
Excitation: 488–561 nm
Emission: 775 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory diseases.
IL-17A is a 32 kDa disulfide-linked homodimer of 136 amino acids, signaling through IL-17R/CDw217. It enhances ICAM-1 expression and stimulates secretion of IL-6, IL-8, G-CSF, and PGE2.
Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating cross-reactivity with the mouse receptor.
IL-17A regulates NF-κB and MAPK pathways, induces COX-2 expression, and enhances nitric oxide production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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