Thermo Fisher Scientific GAD1/GAD67 Monoclonal Antibody (GAD1, 2391)

Applications

Product Specifications

Product Specific Information

• Antibody is tested in direct ELISA.
• For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and catalyze the rate-limiting step in GABA (gamma-aminobutyric acid) synthesis from L-glutamic acid.
Both GADs are expressed in the brain:

• GAD-65 localizes to synaptic vesicle membranes in nerve terminals.
• GAD-67 is distributed throughout the cell and responsible for basal levels of GABA synthesis.
  During increased neurotransmission demand, GAD-65 transiently activates to assist GABA production.
  Loss of GAD-65 impairs GABA neurotransmission, while loss of GAD-67 is lethal.
  Alternative splicing produces two isoforms: the predominant GAD-67 and the minor GAD-25 form.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 없음)