Thermo Fisher Scientific BRAF Monoclonal Antibody (OTI4A5), TrueMAB
인간 BRAF 단백질을 인식하는 마우스 모노클로날 항체로, WB, ELISA, IP에 사용 가능. 동결건조 형태로 제공되며, 1 mg/mL 농도. MAPK/ERK 신호전달 연구 및 암 관련 단백질 분석에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
ELISA
- Tested Dilution: Assay-dependent
Immunoprecipitation (IP)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4A5 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human BRAF expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancers and contributes to postsynaptic responses in hippocampal neurons.
This protein regulates the MAP kinase/ERKs signaling pathway, influencing cell division, differentiation, and secretion.
Mutations in BRAF are associated with various cancers, including:
- Non-Hodgkin lymphoma
- Colorectal cancer
- Malignant melanoma
- Thyroid carcinoma
- Non-small cell lung carcinoma
- Adenocarcinoma of the lung
A pseudogene located on chromosome X has also been identified for BRAF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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