Thermo Fisher Scientific MAP2K4 Monoclonal Antibody (OTI2D5), TrueMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:150

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

2 µg/500 µL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI2D5

Immunogen

Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MAP2K4,uniProtId:P45985-1,ncbiNodeId:9606,antigenRange:1-399,antigenLength:399,antigenImageFileName:CF500430_MAP2K4_P45985-1_House_mouse.svg,antigenImageFileNamePDP:CF500430_MAP2K4_P45985-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

MAP2K4 (MEK4) serine/threonine kinase (mitogen-activated protein kinase 4) MEK4 or MKK4 is a dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38. MAP2K4 preferentially phosphorylates the tyrosine residue of JNKs. MAP2K4 mediates a variety of physiological and pathophysiological processes such as responses to cellular stresses and inflammatory cytokines. MAP2K4 is important for carcinogenesis; a loss-off-function mutation in MAP2K4 was found in lung and pancreatic tumors. Conversely, a pro-oncogenic role was also found for MAP2K4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.