Thermo Fisher Scientific DTL/CDT2 Polyclonal Antibody
DTL/CDT2 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), IP에 적합합니다. 세포주기 조절 및 DNA 복제 관련 연구에 유용하며, 4°C에서 보관하며 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 680 and the C-terminus (residue 730) of human Denticleless Homolog (Chromatin Licensing and DNA Replicating Factor 2). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Target Information
Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response, and translesion DNA synthesis.
The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1).
CDT1 degradation in response to DNA damage ensures proper cell cycle regulation of DNA replication.
CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation controls replication licensing.
Most substrates interact with PCNA via their PIP-box, and those containing the K+4 motif recruit the DCX(DTL) complex, leading to their degradation.
In undamaged proliferating cells, the DCX(DTL) complex also promotes Lys-164 monoubiquitination of PCNA, contributing to PCNA-dependent translesion DNA synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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