Thermo Fisher Scientific GFP Monoclonal Antibody (9F9.F9), DyLight 649
DyLight 649 형광 표지된 GFP 단클론 항체로, Western blot, ICC/IF, ELISA에 사용 가능. 높은 특이성과 감도로 GFP 태그 단백질 검출에 적합. 마우스 IgG1, kappa 형이며 Protein A로 정제된 동결건조 형태. 연구용으로만 사용.
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Thermo Fisher Scientific GFP Monoclonal Antibody (9F9.F9), DyLight 649
Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | >1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9F9.F9 |
| Immunogen | BALB/c mice were immunized with a GFP fusion protein (full-length 246 aa) derived from Aequorea victoria. Hybridoma produced by fusion of BALB/c splenocytes and myeloma cells using conventional hybridoma technology. |
| Conjugate | DyLight™ 649 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- AP
- Biotin
- DyLight 405
- DyLight 488
- DyLight 549
- DyLight 680
- DyLight 800
- FITC
- HRP
- Rhodamine
- Request custom conjugation available
Product Specific Information
- Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below. Avoid freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid. Dilute only before immediate use.
- Immunoelectrophoresis shows a single precipitin arc against anti-Mouse Serum.
- Reactivity observed against recombinant GFP (Aequorea victoria) by Western blot; no reactivity against RFP.
Target Information
The jellyfish Aequorea victoria produces green fluorescent protein (GFP Tag) that emits light during bioluminescence. GFP is widely used as a reporter for gene expression and protein localization in both eukaryotic and prokaryotic systems.
It is a 27 kDa monomeric protein that forms a fluorescent pigment autonomously, absorbing blue light (395 nm) and emitting green light (508 nm).
GFP fluorescence is stable and species-independent, suitable for applications such as protein localization, movement studies, FRET experiments, and yeast two-hybrid interaction assays.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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