Thermo Fisher Scientific Beta III Tubulin Polyclonal Antibody
신경세포 특이적 베타III-튜불린을 인식하는 폴리클로날 항체로, 인간·마우스·랫트 시료에 반응합니다. Western blot과 면역세포염색에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 보관하며 -20°C에서 안정적으로 유지됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Unphosphorylated betaIII-Tubulin synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 444 of human betaIII-Tubulin. This sequence is not found in bI or betaII-Tubulin isotypes, but is well conserved in betaIII-Tubulins from rat and mouse. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | ~0.13 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2943013 |
Product Specific Information
This antibody was cross-adsorbed to phospho-betaIII-Tubulin (Ser-444) peptide before affinity purification using unphosphorylated betaIII-Tubulin (Ser-444) peptide (without carrier).
The antibody detects a 50 kDa protein corresponding to the molecular mass of unphosphorylated betaIII-Tubulin on SDS-PAGE immunoblots of purified brain tubulin and mouse brain tissue.
Target Information
The betaIII-tubulin isoform is dominantly present in cells of neuronal origin and serves as one of the earliest markers of neuronal differentiation.
It is regarded as a specific probe for cells of neuronal origin and for tumors derived from these cells.
BetaIII-tubulin is most abundant in neuronal cells but also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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