Thermo Fisher Scientific Beta III Tubulin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Unphosphorylated betaIII-Tubulin synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 444 of human betaIII-Tubulin. This sequence is not found in bI or betaII-Tubulin isotypes, but is well conserved in betaIII-Tubulins from rat and mouse.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2943013

Product Specific Information

This antibody was cross-adsorbed to phospho-betaIII-Tubulin (Ser-444) peptide before affinity purification using unphosphorylated betaIII-Tubulin (Ser-444) peptide (without carrier). The antibody detects a 50 kDa* protein corresponding to the molecular mass of unphosphorylated betaIII-Tubulin on SDS-PAGE immunoblots of purified brain tubulin and mouse brain tissue.

Target Information

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.