Thermo Fisher Scientific Phospho-VAV2 (Tyr142) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-Vav2 (Tyr-142) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-142 in mouse Vav2. This sequence has high homology with similar regions in rat and human Vav2, and has significant homology to the conserved site in Vav1 and Vav3.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2943015

Product Specific Information

This antibody was cross-adsorbed to unphosphorylated Vav2 (Tyr-142) peptide before affinity purification using phospho-Vav2 (Tyr-142) peptide (without carrier). The antibody detects a 100 kDa* band corresponding to Vav on SDS-PAGE immunoblots of human A431 cells treated with EGF and detects Vav1, Vav2, and Vav3 in Jurkat cells treated with pervanadate. The antibody does not detect these Vavs after treatment with alkaline phosphatase.

Target Information

VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.