Thermo Fisher Scientific Phospho-beta Tubulin (Ser172) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to unphosphorylated betaIII-Tubulin (Ser-172) peptide before affinity purification using phospho-betaIII-Tubulin (Ser-172) peptide (without carrier).
The antibody detects a 50 kDa protein corresponding to the molecular mass of beta-Tubulin on SDS-PAGE immunoblots of purified brain tubulin phosphorylated with ERK2 kinase.

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance.
Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin is also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
Glutamate residues at the C-terminus of beta III tubulin can be glutamylated, though the precise function of such modifications remains unclear.
Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, and this phosphorylation inhibits tubulin incorporation into microtubules.
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of tubulin dimers (alpha and beta subunits, each ~55 kDa) and play key roles in chromosome segregation, intracellular transport, ciliary and flagellar movement, and cytoskeletal structure.
Because beta-tubulin is ubiquitously expressed, it is often used as a loading control in protein detection assays such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.