Thermo Fisher Scientific CD34 (Hematopoietic Stem Cell and Endothelial Marker) Monoclonal Antibody (SPM123)
CD34 단백질을 표적으로 하는 인간 및 원숭이 반응성 단클론 항체로, 줄기세포 및 내피세포 마커 연구에 적합함. IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. 단백질 A/G로 정제된 액상 형태로 4°C에서 보관.
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Thermo Fisher Scientific CD34 (Hematopoietic Stem Cell and Endothelial Marker) Monoclonal Antibody (SPM123)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Cynomolgus monkey, Human, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM123 |
| Immunogen | Detergent solubilized vesicular suspension prepared from human term placenta |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Does not react with Rat, Sheep, Cow or Dog.
Target Information
CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) expressed on many stem cell populations. It functions as a stem cell marker with reversible expression in hematopoietic stem cells. CD34 may act as a receptor involved in adhesion, differentiation, and proliferation of hematopoietic progenitors. It is also implicated in stem cell attachment to the bone marrow extracellular matrix or stromal cells, serving as a scaffold for glycans and facilitating interactions with lectins and selectins. The intracellular domain is phosphorylated by protein kinase C, suggesting a role in signal transduction. CD34 dysfunction is associated with dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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