Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Immunohistochemistry (IHC): Not specified
• Flow Cytometry (Flow): 0.25 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• PE
• Request custom conjugation

Product Specific Information

The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and integrin alpha4beta7 (LPAM-1), playing an important role in lymphocyte and myeloid development.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse bone marrow cells at ≤0.25 µg/test
• Light Sensitivity: Protect from light; tandem dye is photo-sensitive
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days at 4°C in dark conditions with minimal impact on brightness and FRET efficiency.
• Excitation/Emission: 488–561 nm / 775 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration, adhesion, and signal transduction, and may play a role in inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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