
Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), PE-Cyanine7, eBioscience
마우스 CD106(VCAM-1)에 특이적인 429 클론 단클론 항체로, PE-Cyanine7 형광 표지형입니다. Flow cytometry에 적합하며, 0.25 µg/test로 사용됩니다. 4°C 암조건에서 보관하며, 광산화에 민감합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), PE-Cyanine7, eBioscience
Applications and Tested Dilution
- Immunohistochemistry (IHC): Not specified
- Flow Cytometry (Flow): 0.25 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 429 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2784659 |
Additional Formats
Product Specific Information
The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and integrin alpha4beta7 (LPAM-1), playing an important role in lymphocyte and myeloid development.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse bone marrow cells at ≤0.25 µg/test
- Light Sensitivity: Protect from light; tandem dye is photo-sensitive
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days at 4°C in dark conditions with minimal impact on brightness and FRET efficiency.
- Excitation/Emission: 488–561 nm / 775 nm
- Compatible Lasers: Blue, Green, Yellow-Green
Target Information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration, adhesion, and signal transduction, and may play a role in inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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