Thermo Fisher Scientific Alkaline Phosphatase (Placental)/PLAP Recombinant Rabbit Monoclonal Antibody (ALPP, 2899R)
인체 PLAP 단백질을 인식하는 Thermo Fisher의 재조합 토끼 단일클론 항체. WB와 IHC(P)에서 사용 가능하며 높은 특이성과 재현성을 제공. Protein A/G로 정제된 액상 형태로, -20°C 이하에서 보관.
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Thermo Fisher Scientific Alkaline Phosphatase (Placental)/PLAP Recombinant Rabbit Monoclonal Antibody (ALPP, 2899R)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.25–0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ALPP, 2899R |
| Immunogen | Recombinant full-length human ALPP protein (Placental Alkaline Phosphatase, UniProt ID: P05187-1, range 1–535 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Placental Alkaline Phosphatase (PLAP) is involved in regulating inflammatory processes and is one of four related alkaline phosphatases (intestinal, placental, placental-like, liver/bone/kidney).
PLAP reacts with the membrane-bound Regan and Nagao isoenzymes found in the placenta during the third trimester.
It is useful in identifying testicular germ cell tumors and distinguishing PLAP-positive somatic cell tumors expressing epithelial membrane antigen (EMA).
Although proposed to play a role in matrix mineralization, mice lacking functional PLAP show normal skeletal development.
PLAP has been associated with hypophosphatasia, a disorder characterized by hypercalcemia and skeletal defects, with variability depending on mutation type and onset.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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