Thermo Fisher Scientific ATP Citrate Lyase Polyclonal Antibody
ATP Citrate Lyase 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal antibody로, WB, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피 정제, 액상 형태, 4°C 또는 -20°C 보관.
- 카탈로그번호
- PA5119638
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within human ATP citrate lyase aa 813–1066 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2913211 |
Product Specific Information
- Positive Control: NIH-3T3, A549, SH-SY5Y, mouse pancreas tissue lysate, rat brain tissue, human colon cancer tissue, human pancreas tissue, mouse testis tissue
- Subcellular Location: Cytoplasm, Nucleus
Target Information
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (approximate molecular weight 440,000) composed of identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with the hydrolysis of ATP to ADP and phosphate. Acetyl-CoA serves several biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in acetylcholine biosynthesis. Two transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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