Thermo Fisher Scientific IGF2BP3 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10–20 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is prostate carcinoma or placenta tissue.

Target Information

Insulin-like growth factor-II messenger RNA (mRNA)-binding protein-3 (IMP-3), also known as K homology domain-containing protein overexpressed in cancer (KOC) and L523S, is a member of the insulin-like growth factor-II mRNA-binding protein family.
It is expressed during embryogenesis and in certain malignancies. IMP-3 is expressed in malignant melanoma but not in benign nevi, even when dysplastic features are present.
IMP-3 shows higher expression in melanomas than Spitz nevi and is significantly more expressed in metastatic melanomas compared to thin melanomas.
IMP-3 may be involved in the progression of malignant melanoma and play an important role in regulating the biological behavior of this tumor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.