Thermo Fisher Scientific Cytokeratin 20 Polyclonal Antibody
사이토케라틴 20 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC에 적합합니다. 인간 시료 반응성이 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 PBS(pH 7.6, 1% BSA) 내 보관하며, -20°C에서 냉동 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to C-terminus of human keratin 20 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2549933 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with the primary antibody for 10 minutes at room temperature.
A suggested positive control is colon adenocarcinoma tissue.
Target Information
The protein encoded by this gene is a member of the keratin family. Keratins are intermediate filament proteins responsible for maintaining the structural integrity of epithelial cells.
They are divided into cytokeratins and hair keratins. Type I cytokeratins are acidic proteins arranged in heterotypic pairs with type II keratins.
Cytokeratin 20 is a major cellular protein in mature enterocytes and goblet cells, specifically expressed in gastric and intestinal mucosa.
Type I cytokeratin genes are clustered in the chromosome region 17q12–q21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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